| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Matthew Z (10)
|
MAHAC |
38.88 |
38.76 |
-0.12 |
| 2 |
Zagaceta, Magill M (9)
|
MAHAC |
38.53 |
39.30 |
0.77 |
| 3 |
M'Ikanatha, Isaac (10)
|
MAHAC |
40.90 |
40.96 |
0.06 |
| 4 |
George, Kiran L (9)
|
MADSC |
46.91 |
46.93 |
0.02 |
| 5 |
Tsang, Jonathan M (10)
|
MADSC |
53.25 |
47.44 |
-5.81 |
| 6 |
Wingert, Markus J (9)
|
MAHAC |
51.75 |
50.43 |
-1.32 |
| 7 |
Bu, Tianshi J (9)
|
MAHAC |
51.27 |
50.92 |
-0.35 |
| 8 |
Mailman, Matthew H (10)
|
MAHAC |
52.49 |
52.04 |
-0.45 |
| 9 |
Serfass, Caleb P (9)
|
MAHAC |
56.31 |
54.22 |
-2.09 |
| 10 |
Fastrich, Owen S (9)
|
MAHAC |
1:08.40 |
1:00.87 |
-7.53 |
| 11 |
Nguyen, Kyle A (9)
|
MAHAC |
|
1:01.86 |
- |
|
Buckwalter, Darius H (10)
|
MALAC |
|
DQ |
- |
|
Wiggins, Maximilian L (9)
|
MALAC |
|
DQ |
- |
|
Meyers, Joshua C (9)
|
MADSC |
|
NS |
- |
|
Yan, James D (9)
|
MADSC |
1:01.43 |
NS |
- |